Figure 7
From: Characterization of the multimeric structure of poly(A)-binding protein on a poly(A) tail
Effects of Lt2 deletion in PABPC1 on cap/poly(A)-dependent translation. (a) Translational enhancement by the cap and poly(A) tail. The relative increase of translation activity is shown as the fold increase in the luciferase activity of the mRNA without cap and poly(A). (b) Western blotting against PABPC1 for the control RRL and the PABPC1-depleted RRL. The control RRL was treated with GST immobilized glutathione-sepharose beads. The uncropped original blots were shown in Supplementary Figure 6. (c,d) Translation activity of the capped luciferase mRNA with (c) or without (d) poly(A) in the PABPC1-depleted RRL in the presence of the purified PABPC1, PABPC1ΔLt2 or PABPC1ΔLt4 (10 nM). Translation activity is shown as a percentage of the luciferase activity in the control RRL. The results represent average values and standard deviations in three independent experiments. *and N.S. indicate P < 0.05 and not significant in statistical analysis, respectively.
