Figure 1 | Scientific Reports

Figure 1

From: Transcriptome Analysis in Spleen Reveals Differential Regulation of Response to Newcastle Disease Virus in Two Chicken Lines

Figure 1

Validation analysis by Fluidigm Biomark assay of log2 fold change (Log2FC) of selected genes that were significant for different contrasts in RNA-seq analysis. Contrasts between different line by treatment combinations are marked in different colors and each combination is labeled as Line_Treatment (FA: Fayoumi, LE: Leghorn, CT: Control, NDV: Newcastle disease virus). Pearson correlation coefficient is labeled as “r”. Log2FC in Biomark assay equals −ΔΔCt for each comparison. Average cycle threshold (Ct) value for each group is the mean of samples in that group. Average expression of three housekeeping genes including GAPDH, ACTB and HPRT1 were used for normalization of Ct values.

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