Figure 5
From: Neuroprotective Drug for Nerve Trauma Revealed Using Artificial Intelligence
NeuroHeal accelerates nerve regeneration and improves muscle reinnervation and functional recovery after nerve crush injury. (A) Left panels, mean amplitudes of CMAP from ipsilateral gastrocnemius and plantar muscles after sciatic nerve crush of animals treated with vehicle (Veh) or NeuroHeal (C1; n = 5, ANOVA, post hoc Bonferroni*p < 0.05 vs. Veh). Right panels, representative recordings. (B) Histogram of the percentages of treated animals that presented electrophysiological evidence of reinnervation at the plantar muscle at different time-points. (C) Left, Plot of the sciatic functional index (SFI) obtained with walking track analysis of sciatic nerve in RA animals treated with either vehicle (Veh) or NeuroHeal (C1). Right, Representative footprints from ipsi- and contralateral paws at 35 days post injury (dpi). (D) Left, bar graph showing the percentage of reinnervated motor endplates at plantar muscle. Right, representative pictures of reinnervated neuromuscular junctions showing nerve fibers immunostained by NF200 (red) and end-plates labeled with bungarotoxin (green). (n = 4). (E) Microphotographs of spinal MNs stained with Nissl green at the ventral horn showing no signs of cell death due to nerve crush at 3 weeks post-injury.
