Figure 6
From: Neuroprotective Drug for Nerve Trauma Revealed Using Artificial Intelligence
Molecular targets of NeuroHeal. (A) List of seed proteins predicted to be key synergic targets in the action of NeuroHeal. (B) Representation of putative NeuroHeal MoA from initial ACA and RIB targets to downstream possible effects to yield the synergic effects (pink) through its targets (orange). Representation is based on analysis using STRING and IntAct platforms and manual scrutiny of relevant literature. (C) Left, microphotographs of ipsilateral (I) and contralateral (C) ventral horns immunostained to reveal Itgb1, Kif5c, and SIRT1 in MNs (red) counterstained with green fluorescent Nissl (merged pictures) in animals treated with vehicle (Veh) or NeuroHeal (C1). Scale bar = 50 μm. Right and Bottom, bar graphs of the average ratios of immunofluorescence intensity (IF) between ipsi- and contralateral sides within an equivalent pre-determined region of interest (ROI) localized in the lateral grey matter for all conditions except for DCTN1, which was measured in the white matter (n = 4 animals, 5 MN/section, 3 sections, *p < 0.05). The bottom right histogram of DCTN1 analysis shows the quantification of total integrated intensity on each side to document that NeuroHeal only changes expression on the injured site (n = 4 animals, 5 MN/section, 3 sections, *p < 0.05).
