Figure 7
From: Neuroprotective Drug for Nerve Trauma Revealed Using Artificial Intelligence
SIRT1 overexpression promotes MN survival after RA. (A) Top, representative microphotographs of SIRT1 immunolabelling (red) in infected MNs in control animals (upper) and RA injured animals (lower) treated with either AAVrh10-GFP or AAVrh10-SIRT1 and counterstained with green fluorescent Nissl. Scale bar = 100 µm (top); 25 µm (bottom). Bottom, histogram of cytosolic versus nuclear SIRT1 localization in the RA-injured MNs 21dpi after damage. (B) Histogram of the percentage of avulsed MNs with high nuclear immunofluorescence intensity for each acetylated form of either H3 (H3-K9) or p53 (p53-K373) at the ipsilateral side of RA animals infected with either vector (n = 4, *p < 0.05 vs. AAV-GFP). (C) Representative microphotographs of Nissl-labelled MNs (green) at the ipsilateral ventral horns of RA animals treated with either AAVrh10-GFP or AAVrh10-SIRT1and histogram of MN survival ± SEM expressed as % of MN on the contralateral side (contra) (n = 4, *p < 0.05 vs. AAVrh10-GFP).
