Figure 2 | Scientific Reports

Figure 2

From: Establishment and analysis of a novel mouse line carrying a conditional knockin allele of a cancer-specific FBXW7 mutation

Figure 2

Cre-mediated gene recombination and expression of Fbxw7R468C transcripts. (A) PCR genotyping of DNA from E18.5 embryos. A male Fbxw7LSL-R468C/+ mouse was crossed with a female CAG-Cre mouse. The mutant Fbxw7R468C allele (R468C) corresponded to a band of 384 bp and the wild-type allele (WT) to a band of 338 bp. Lane 1: Fbxw7R468C/+, lane 2: Fbxw7R468C/+;CAG-Cre, lane 3: Fbxw7+/+, and lane 4: Fbxw7+/+;CAG-Cre. (B) PCR direct sequence analysis of RT-PCR products from Fbxw7+/+ and Fbxw7R468C/+ mice. RNA was extracted from mouse embryonic fibroblasts (MEFs) isolated from E12.5 Fbxw7+/+ and Fbxw7R468C/+ embryos.

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