Figure 5 | Scientific Reports

Figure 5

From: Production of Wilson Disease Model Rabbits with Homology-Directed Precision Point Mutations in the ATP7B Gene Using the CRISPR/Cas9 System

Figure 5

Results of genotype and sequencing chromatograms of F1 pups from different parents. (A) The analysis of sequences of F1 pups obtained from mating wild rabbits with F0–48 and F0–24. The point mutation is underlined in magenta, and the endonuclease cutting site Bln I is underlined in blue. The mutated base numbers and their sequences are shown on the right (−, deletion). A > G indicates that G was substituted by A, and T > G indicates that G was substituted by T. WT, wild type; KO, knock-out; KI, knock-in (point mutation). (B) Sequencing chromatogram of F1 obtained from F0–48 mated with a wild-type rabbit. The black arrows represent the sites of synonymous mutations and point mutations. (C) Sequencing chromatogram of an F1 pup obtained from F0–24 mated with wild-type rabbits. (D) The analysis of sequences of F1 obtained from F0–47 mated with F0–24. The endonuclease cutting site Bln I is underlined in blue. The mutated base numbers and their sequences are shown on the right (−, deletion). A > G indicates that G was substituted by A. WT, wild type; KO, knock-out; SYNMUT, synonymous mutation.

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