Table 1 The results of mutation pups obtained by microinjection at different times.

From: Production of Wilson Disease Model Rabbits with Homology-Directed Precision Point Mutations in the ATP7B Gene Using the CRISPR/Cas9 System

 

Zygotes microinjected

pups obtained (%transferred)

Pups with KO (% pups obtained)

pups with KI (% pups obtained)

Zygotes injected 19 hr after HCG treatment

62

14 (22.58%)

7 (50%)

2 (14.29%)a

Zygotes injected 14 hr after HCG treatment

56

17 (30.36%)

5 (29.41%)

9 (52.94%)b

  1. KO: knock-out, KI: knock-in (point mutation).
  2. Chi-square analysis demonstrated significant differences between a and b (*P < 0.05).
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