Figure 1 | Scientific Reports

Figure 1

From: Molecular engineering of antibodies for site-specific covalent conjugation using CRISPR/Cas9

Figure 1

Illustration of CRISPR/Cas9 genome editing approach of hybridoma cells for site-specific modification of antibodies. Hybridoma cells were modified by co-transfection with plasmid expressing sgRNA and Cas9, and linearized HDR repair plasmid. After 48 hrs, GFP-positive cells were isolated using fluorescence-activated cell sorting (FACS) into 96 well plates and cultured. Clones were analyzed for incorporation if inserted tag by gel and sequence analysis.

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