Figure 1
From: Dysregulated fibronectin trafficking by Hsp90 inhibition restricts prostate cancer cell invasion
The Hsp90 inhibitor, AUY922, selectively alters cellular pathways involved in the cytoskeleton and ECM. (A) KEGG pathways determined by over-representation analysis of differentially expressed genes identified through RNA-seq analysis of AUY922 treated PDEs (48 h: 500 nM) using the InnateDB database. A barcode plot and heatmap of RNA-seq gene expression data from regulation of actin cytoskeleton pathway for individual explants is shown, and significant changes in gene expression were validated in independent explants by qRT-PCR (ANXA2 & AURKB shown). (B) KEGG pathways determined by the Database for Annotation, Visualization and Integrated Discovery (DAVID) using differentially expressed proteins identified through proteomic analysis of AUY922 treated PDEs 48 h: 500 nM, n = 12). Proteins identified by 2D-DIGE proteomics analysis were validated in independent PDE samples by Western blot. ERK1 was used as loading control. Full-length blots are presented in Supplementary Figure 5.
