Figure 6
Cardiac fibroblasts differentiation into myofibroblasts. (A) Gene expressions of αSMA, CTGF and Collagen 1A1; (B,C) immunostaining of αSMA; and (D) percentage of αSMA+ myofibroblasts. Cardiac fibroblasts were seeded in collagen gels. The Gel and Gel/O2 groups were injected into the collagen gels respectively and cultured under hypoxia (1% O2). Gene and protein expressions were measured 2 days after injection. TGFβ1 (5 ng/mL) was added into the medium during culture. *p < 0.05. Scale bar = 30 µm.
