Table 1 Primers used for real time RT-PCR.
Primer | Sense | Sequence | Tm(°C) |
|---|---|---|---|
β-actin | Forward | 5′-ACTCTGTGTGGATTGGTGGC-3′ | 60 |
Reverse | 5′-AGCTCAGTAACAGTCCGCCT-3′ | 61 | |
αSMA | Forward | 5′-CATCAGGAACCTCGAGAAGC-3′ | 58 |
Reverse | 5′-TCGGATACTTCAGGGTCAGG-3′ | 58 | |
CTGF | Forward | 5′-TTCACCTACAGCACGCTTGT-3′ | 60 |
Reverse | 5′-TTGGGATGGAGGGAGTTTAC-3′ | 57 | |
Collagen 1A1 | Forward | 5′-ACTGGTATTTGCAACTGCTTTGG-3′ | 60 |
Reverse | 5′-GCGACCCACACAAGGGTCT-3′ | 56 | |
TGFβ1 | Forward | 5′-GCGCCTGCAGAGATTCAAGTCAAC-3′ | 65 |
Reverse | 5′-GTATCAGTGGGGGTCAGCAGCC-3′ | 65 | |
TGFβRII | Forward | GACCAGAAATTCCCAGCTTCT | 58 |
Reverse | CAACGTCTCACACACCATCTG | 59 |
