Figure 4 | Scientific Reports

Figure 4

From: Clathrin heavy chain 22 contributes to the control of neuropeptide degradation and secretion during neuronal development

Figure 4

CHC22 loss induces NPY accumulation. (A) Quantitative RT PCR analysis of the mRNA levels of NPY, SCG2, VGF and SCG3 in cells treated with CHC22 knockdown, normalised to GAPDH. Note an upregulation of SCG2 and no effect on NPY levels. Statistical tests are one-sample T-test on log transformed proportion data. (B) Western blot of whole cell lysates demonstrating knockdown of CHC22 by two different siRNAs caused accumulation of NPY at the protein level (C) Knockdown of CHC17 has no effect on NPY levels, whereas CHC22 knockdown induces significant accumulation. (D) Quantification of NPY levels from three independent repeats. (E) Overexpression of Wild type CHC22, but not mutant p.E330K CHC22 rescues the accumulation of NPY on CHC22 knckdown. Figure complied from three separate blots. (F) Quantification of NPY levels from at least three independent repeats. Statistics represent one way ANOVA with Dunnett’s multiple comparison post hoc test (p < 0.05, p < 0.01 p < 0.001 are *, **, *** Respectively).

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