Figure 3

Comparison of 2D SMLM images of Alexa-647 labeled microtubules in U2OS cells acquired with an ORCA-Flash4.0 camera and a GS3-U3-51S5M-C camera. (a) Overlay of the two images (red - ORCA-Flash4.0, green - GS3-U3-51S5M-C). (b) Zoom in of the area white boxed area in (a), ORCA-Flash4.0 camera. (c) Zoom in of the white boxed area in (a), GS3-U3-51S5M-C camera. (d) Histograms of the white boxed areas in (b,c) for the two cameras. Scale bars are 2 μm in (a), 200 nm in (b,c).