Figure 4
3D SMLM image of Alexa-647 labeled microtubules in U2OS cells taken using two GS3-U3-51S5M-C cameras in a biplane configuration. (a) 3D SMLM image with Z color scale as shown in the color bar. (b) Zoom in of the area white boxed area in (a) with the same Z color scale. (c) An X/Z cross section of the microtubule in the white boxed area in (b). Gaussian fits of the width of microtubule in (c) give σ = 23.6 nm in X,Y and 22.3 nm in Z. Scale bars are 5 μm in (a), 500 nm in (b), 50 nm in (c).
