Figure 9

PAFB and PAF reduced CPE of HCoV 229E in L132 cells. PAFB and PAF (8 µM, respectively) were applied. (a) Crystal violet assay visualized the reduction of virus-induced CPE at MOI 0.01. (b) Reduction of the viral CPE at MOI 1, MOI 0.1 and MOI 0.01 at 72 h post infection in the presence of PAFB and PAF (0, untreated control). (c) Immunostaining of HCoV protein in L132 cells with mouse-anti hexon antibody and goat anti-mouse FITC-labelled secondary antibody after treatment with PAFB and PAF. Nuclei of fixed L132 cells were counter-stained with Hoechst 33342. Virus-uninfected cells (Mock) served as a negative control and virus infected cells without protein treatment as positive control (HCoV 229E); *p < 0.05; **p < 0.01.