Figure 1
The transcript levels of NRT1.1 in the nlp7 plants were reduced when NH4+ is present. (a) Relative expression of NRT1.1 in WT and the nlp7 plants. Seedlings were planted on medium containing 10 mM potassium nitrate or 10 mM ammonium nitrate for 7d for testing NRT1.1′s expression. Error bars mean ± SD of four biological replicates. The significant difference (p < 0.05, t test) is indicated by different letters. (b) Relative expression of NLP7 in WT and the chl1 mutants. The growth conditions of plants are the same as (a). Error bars mean ± SD of four biological replicates. (c) Nitrate response of NRT1.1 in WT and the nlp7 mutants. Seedlings were planted on medium with 2.5 mM ammonium succinate for 7d, then treated with 10 mM KNO3 or KCl for 2 h followed by collecting samples for detecting nitrate induction of NRT1.1. Error bars mean ± SD of four biological replicates. The significant difference (p < 0.05, t test) is indicated by different letters.
