Figure 2
NLP7 functions in the same nitrate regulation pathway as NRT1.1. (a) Root YFP observation of WT, chl1-13, nlp7-4, and chl1-13 nlp7-4 plants. Fluorescence and light images of seedlings which grown on the medium with 10 mM ammonium nitrate for 5d were captured using a fluorescence microscope. (b) Quantification of root YFP signal levels of WT, chl1-13, nlp7-4, and chl1-13 nlp7-4 plants. The same conditions for plants growth were used as (a). Error bars mean ± SD of sixty biological replicates. The significant difference (p < 0.05, t test) is indicated by different letters. (c) Nitrate content in WT, chl1, nlp7, and chl1-13 nlp7-4 plants. Seedlings grown on the medium with 10 mM ammonium nitrate for 7d were sampled for nitrate content detection. Error bars mean ± SD of four biological replicates. The significant difference (p < 0.05, t test) is indicated by different letters. FW, fresh weight. (d) Nitrate reductase activity in WT, chl1, nlp7, and chl1-13 nlp7-4 plants. The same growth conditions were used as (c). Error bars mean ± SD of four biological replicates. The significant difference (p < 0.05, t test) is indicated by different letters. FW, fresh weight. (e) and (f) Nitrate accumulation in WT, nlp7, chl1-13 and chl1-13 nlp7-4 plants. Seedlings were planted on the medium with 2.5 mM ammonium succinate for 7 d followed by the treatments with different KNO3 concentrations for 2 h (e) or with 5 mM KNO3 for different times (f). The whole plants were used for nitrate content test. Error bars mean ± SD of four biological replicates. The significant difference (p < 0.05, t test) is indicated by different letters.
