Figure 4
From: Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency
Differentiation properties of RICs. (a–c) Ectodermal neurons in RICs were detected using antibodies against the neural markers Tuj1, neurofilament (NF), and MAP2. (d,e) Staining with antibodies against the cardiomyocyte markers NKX2-5 and TNNT2. (f) Staining for the hepatocyte marker Hnf4α. (g) Oil Red O staining of hepatocytes induced from HDFs. (h) Testis injected with RICs. (i) RICs obtained from MEFs. (j) Oil Red O staining of hepatocytes induced from MEFs. All fluorescence images of nuclei were obtained from cells stained with Hoechst 33342 (blue). Bars = 100 µm (a–g,i and j), 1 mm (h).
