Figure 5
From: Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency
Cell cycle and mesenchymal properties of RICs. (a–c) Immunocytochemistry of RICs performed using (a) anti-single-strand DNA, (b) anti-CDC27, and (c) anti-β-tubulin antibodies. (d) Cell redox staining analysis. Small windows show colorimetric intensity ratio. (e) Cell cycle analysis of Fucci2-harboring MEFs. See also Video S3a,b. (f–p) Immunocytochemistry of RICs and normal HDFs performed using antibodies against the adhesion molecules E-cadherin (f,g) and vimentin (h,i). Epithelial–mesenchymal transition (EMT) markers are indicated as TWIST1 (j,k), SNAIL1 (l,m), and merged (n,o). Nuclei were stained with Hoechst 33342 (blue). Bars = 100 µm (a–c,e,h,j,l,n), 50 µm (g,i,k,m,o), 20 µm (f).
