Figure 3
(a) Typical bright-field image of an injected sample plug (Red dye) at the tapered tip of a 50 μm i.d. capillary after translational spontaneous injection; (b) Typical fluorescence image of an injected sample plug of sodium fluorescein at the tapered tip of a 50 μm i.d. capillary after translational spontaneous injection; (c) Typical electropherogram of FITC-labeled amino acids; Conditions: sample, mixture of 5 μM FITC-labeled arginine, phenylalanine and glycine; working electrolyte, 5 mM borate buffer solution (pH 9.2); fused-silica capillary, 50 μm i.d., 3.8 cm total length, 2.6 cm effective separation length; translational spontaneous injection mode; separation field strength, 800 V/cm. (d) Electropherogram of 11 continuous separations of three FITC-labeled amino acids. Conditions are the same as (c).
