Figure 4
(a) Electropherogram of a mixture of amino acid enantiomers under the MEKC mode. Conditions: sample, mixture of 2 μM FITC-labeled D-leucine and L-leucine, D-aspartate and L- aspartate; working electrolyte, 5 mM borate buffer solution (pH 9.2) with 8 mM β-CD and 12 mM STC; translational spontaneous sample injection mode; separation electric field, 700 V/cm; (b) Electropherogram of three proteins under the CGE mode. Conditions: sample, mixture of 1.67 mg/mL FITC-labeled myoglonbin, albumin and BSA; translational spontaneous sample injection mode; separation electric field, 300 V/cm; (c) Electropherogram of a DNA ladder under the CGE mode; Conditions: sample, 1 μg/mL DNA ladder (50, 150, 300, 500, 766 bp), labeled with SYBR Green II; working electrolyte, 1 × TBE buffer, 6% PVP (m/v); electrokinetic injection mode; injection electric field, 50 V/cm; injection time, 0.5 s; separation electric field, 250 V/cm.
