Figure 5 | Scientific Reports

Figure 5

From: A Low-Cost Palmtop High-Speed Capillary Electrophoresis Bioanalyzer with Laser Induced Fluorescence Detection

Figure 5

Electropherograms of PCR products (a) and RFLP digestion products (b) of KRAS proto-oncogenes in three types of CRC cells. Conditions: samples, SW480, HT29 and CCL-187 cells with KRAS gene statuses of mutant, wild-type, and heterozygote; sample DNA fragments were labeled with SYBR Green II, with 200 bp DNA fragment added as internal standard; working electrolyte, 1 × TBE buffer, 6% PVP (m/v); electrokinetic injection mode; injection electric field, 200 V/cm; injection time, 3 s; separation electric field, 200 V/cm.

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