Figure 2

EpCAM expression in mid-gestation to perinatal mouse embryos. (a) EpCAM expression was detected by immunohistochemistry staining (brown) in sections of cryo-conserved C57BL-6 embryos at gestation day E9.5 (scale bar represents 200 µm), E12.5 and E18.5, as indicated. Cytoplasm and nucleus were counterstained with H&E. (b) Magnification of EpCAM expression in embryos at E9.5 (scale bar represents 100 µm), E12.5 (scale bar represents 200 µm) and E18.5 (scale bar represents 200 µm). (c) EpCAM, Foxa2, vimentin and nestin protein expression is depicted in consecutive sections of E9.5 embryos (scale bars represent 100 µm). C colon; E eye; ENT ear nose and throat area; FB fore-brain; H heart; HB hind-brain; HP heart primordium; K kidney; L lung; LB limb bud; LP liver primordium; MB mid-brain; OP otic pit; P periderm; PE pharyngeal epithelium; PG primitive gut; So somite.