Figure 1
Effect of chronic hypoxia and/or high glucose on Neuro-2a cell viability. (a) Measurement of Neuro-2a cell proliferation. Neuro-2a cells were exposed to hypoxia and/or high glucose for up to 48 h. “C” represented control conditions (25 mM glucose), “G” represented high glucose conditions (75 mM glucose). Cell proliferation was determined by the MTS assay. (b,c) Western blot analysis of cleaved caspase-3 expression in Neuro-2a cells. The ImageJ densitometric analysis of the cleaved caspase-3 from immunoblots is shown. The results were expressed as the mean ± SEM from three independent experiments. Two-way ANOVA, **p < 0.01, Hypoxia vs Normoxia. Full-length blots are presented in Supplementary Fig. S2.
