Figure 5
Inhibition of autophagy degradation induced cell death. (a) An MTS assay was used to access Neuro-2a cell proliferation under hypoxia and/or normoxia in the presence or absence of BAF. Treatment with BAF induced a decreased OD value under normoxia but did not further change the OD value under hypoxia. “C” represented control conditions (25 mM glucose), “G” represented high glucose conditions (75 mM glucose). (b) Immunoblots were performed to determine the level of the cleaved caspase-3 protein in Neuro-2a cells. BAF treatment induced an increase in the level of cleaved caspase-3 protein under normoxia but did not further change the expression of cleaved caspase-3 under hypoxia. The results are expressed as the mean ± SEM from three independent experiments. Two-way ANOVA, **p < 0.01 treated vs. untreated. Full-length blots are presented in Supplementary Fig. S2.
