Figure 6
Chronic hypoxia triggered a loss of mitochondrial potential and destruction of the mitochondrial structure. (a) Neuro-2a cells were subjected to hypoxia and/or high glucose for up to 48 h. The mitochondria potential was measured by JC1 staining. Quantitative analysis by flow cytometry revealed that chronic hypoxia lowered the ratio of the red and green fluorescence, while high glucose did not change it with or without hypoxia. “C” represented control conditions (25 mM glucose), “G” represented high glucose conditions (75 mM glucose). The results are expressed as the mean ± SEM from three independent experiments. Two-way ANOVA, **p < 0.01, Hypoxia vs Normoxia. (b) Representative examples of mitochondrial morphology under hypoxia and/or high glucose examined via TEM. M indicate mitochondria; L represent lipid droplets. Scale bar: 0.5 μm.
