Figure 8
BNIP3-mediated mitophagy was insufficient during hypoxia. Neuro-2a cells were incubated for 48 h in each experiment, then fixed and immunostained with the LC3B, BNIP3, and COXIV antibodies. Cells were counterstained with DAPI to visualize nuclei. (a) Immunodetection of endogenous BNIP3 (green) colocalized with the mitochondrial marker COXIV (red), where it appeared as yellow. Scale bar: 50 μm. (b) Less colocalization of LC3B (green) and COXIV (red) was detected by fluorescence microscope. Scale bar: 50 μm. (c) Less colocalization of LC3B (green) and BNIP3 (red) was detected by fluorescence microscope. Scale bar: 50 μm.
