Figure 9
The accumulation of damaged mitochondria under hypoxia was independent of autophagy impairment. (a) Neuro-2a cells were submitted to hypoxia and/or high glucose for up to 42 h and then subjected to 10 nM BAF for an additional 6 h. Immunoblots were performed to determine the levels of BNIP3 and TOMM20. (b) The ImageJ densitometric analysis from immunoblots is shown. “C” represented control conditions (25 mM glucose), “G” represented high glucose conditions (75 mM glucose). The results are expressed as the mean ± SEM from three independent experiments. Two-way ANOVA, **p < 0.01, *p < 0.05, Hypoxia vs Normoxia. Full-length blots are presented in Supplementary Fig. S2.
