Figure 3 | Scientific Reports

Figure 3

From: A novel PAX7 10-bp indel variant modulates promoter activity, gene expression and contributes to different phenotypes of Chinese cattle

Figure 3

PAX7 promoter activity modulated by the 10-bp indel polymorphism. (A) Luciferase activity in C2C12 cells transfected with recombinant plasmids containing serial promoter fragments (pGL3-pro1, pGL3-pro2, pGL3-pro3, pGL3-pro4, and pGL3-pro5) of the Pax7 promoter. The backbone vector pGL3-Basic was used as a negative control. PpGL3-pro2 vs. pGL3-pro1 = 0.0118, PpGL3-pro2 vs. pGL3-pro3 = 0.0042, PpGL3-pro2 vs. pGL3-pro4 = 0.0495, PpGL3-pro2 vs. pGL3-pro5 = 0.0241. (B) Luciferase activity in C2C12 cells transfected with recombinant plasmids containing the Ins-Ins or Del-Del genotype. pcDNA3.1-ZNF219 was transiently co-transfected into C2C12 cells together with the reporter vectors. Each experiment was repeated at least three times. The data were mean ± standard error (S.E.) of the normalized luciferase activity. *P < 0.05 represents a significant difference.

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