Figure 5
Effect of FliH and FliI deletion on the number and length of the flagellar filaments. (a) Fluorescent images of SJW1103 (WT), NH004 (∆fliHI flhB* ∆flhA), MMHI0117 (∆fliHI flhB*), MMHI0117-1 [∆fliHI flhB* flhA(F459A)], MMB017 (flhB*) and MMA459 [flhA(F459A)]. The flagellar filaments were labeled with Alexa Fluor 594. The fluorescence images of the filaments labeled with Alexa Fluor 594 (red) were merged with the bright field images of the cell bodies. (b) Distribution of the number of the flagellar filaments. The filaments were counted for more than 200 cells of each strain. (c) Measurements of the length of the flagellar filaments. Filament length is the average of more than 100 cells for each strain, and vertical lines are standard deviations.
