Figure 2
Regulation of the Hippo pathway in salmon maturation. (A) Regulation of the Hippo pathway genes (gene names shown in Supplementary Fig. S5) in testis from prepubertal to pubertal stages, inferred by RNA-seq with Male experiment 1. Green and blue indicate up and down-regulation, respectively, and white colour means not regulated. Fold change of gene expression for selected Hippo genes measured by qPCR for (B) prepubertal to maturing testis from Male experiment 2 (n = 15 in each group, and n = 8 for vgll321 in the maturing group), (C) prepubertal to testis regressing from maturity from Male experiment 3 (n = 8–10 in each group), and (D) from oildrop (prepubertal) to early vitellogenic (early puberty) stages in ovaries from the Female experiment (n = 10–13 in each group). Error bars show SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
