Figure 3
In situ hybridization shows localization of vgll3 in gonads. (A) HES staining tissue sections of prepubertal testis, (B) expression of vgll3 in Sertoli cells in prepubertal testis, with negative control shown in the upper right corner. (C) The germ cell specific gene piwi used as a germ cell marker by in situ hybridization on an immature testis, with negative control shown in the upper right corner. (D) HES staining tissue sections of germ cell free testis, (E) expression of vgll3 in germ cell free testis, showing expression in Sertoli cells, and (F) negative control. (G) HES staining tissue sections of vitellogenic ovary, (H) expression of vgll3 in granulosa cells in vitellogenic ovary, and (I) negative control. The scale bars in (A,B,D–I) is 30 µm, and 50 µm in (C). Annotations show areas with Sertoli cells in contact with type A spermatogonia (arrowheads) and other Sertoli cells (arrows), and areas within spermatogenic tubules with little or no labelling (stippled lines).
