Figure 1
From: Large-scale intact glycopeptide identification by Mascot database search
HCD-MS2 spectrum of a di-sialylated bi-antennary glycopeptide (m/z 1177.81373+) derived from bovine alpha-1-acid glycoprotein 1. The glycopeptide was fragmented at an NCE value of 15. (A) Y n represent the peptide bound glycosidic cleavage ions and the insert shows the corresponding peptide bound glycan structures. (B) Linearizing glycan structures with the corresponding three letters O (GlcNAc, GalNAc), J (Galactose, Mannose) and U (Neu5Ac) are depicted. A linearized di-sialylated bi-antennary glycan structure attached to the N-terminus of a peptide and annotated y and b type cleavage ions corresponds to the MS2 spectrum.
