Figure 2
From: Large-scale intact glycopeptide identification by Mascot database search
Mascot annotated MS2 spectra of a di-sialylated bi-antennary N-glycopeptide (m/z 1177.81373+) fragmented at different NCE values. NCE values of (A) 15, (B) 25, (C) 35 and (D) the composite MS2 spectrum of the same precursor fragmented using the stepped NCE values of 15, 25 and 35 are displayed. Standard bovine alpha-1-acid glycoprotein 1 was digested with trypsin, the glycopeptides were analyzed by LC-MS and the data were searched against the custom glycoprotein database using the Mascot search engine.
