Figure 5
From: Large-scale intact glycopeptide identification by Mascot database search

Annotation of nine different glycan structures with varied degree of complexity and sialylation by Mascot on a single glycosylation site (Asn 93) of alpha-1-acid glycoprotein 1 in serum. Shown here are the representative HCD MS2 spectra annotated by Mascot. The nine different glycopeptide variants included the mono-sialylated bi- (A), tri- (B), tetra-antennary (C), and the di-sialylated bi- (D), tri- (E), tetra-antennary (F). Tri- (G,H) and tetra-sialylated (I) glycan structures on the same glycosylation site were also annotated by Mascot.