Figure 2

Phylogenetic relationships of the EV-B69 study strain 15_491 and other EV-B prototype strains. The phylogenetic trees were performed from the nucleotide sequence alignment of a ~2560, 1733 and 2268 bp-sized nucleotide sequence data within the P1 (a), P2 (b), and P3 (c) coding regions, respectively, by using the neighbor-joining algorithm of the MEGA 5.0. software. The reliability of tree topology was estimated by using 1000 bootstrap replicates. Bootstrap values are indicated if higher than 80%. Scale bar represents nucleotide substitutions per site. Black triangles indicate the strains from this study. Black circle indicates prototype strain. GenBank accession numbers for published sequences are shown in the tree.