Figure 2
Withdrawal from long term alcohol exposure increases glymphatic function. (A) Timeline diagram of experiment. Arrows on the bottom indicate injections of alcohol (EtOH) or saline. This experiment was performed 15 minutes after the last of 30 days of daily injections. (B) Tracer influx in the cortex 30 minutes after cisterna magna injections of Alexa-647-conjugated bovine serum albumin (BSA-647) in awake mice with chronic alcohol exposure. Scale bars: 100 μm. (C) Area covered by tracer influx in coronal brain slices 30 minutes after cisterna magna injection. X-axis indicates the distance to bregma. 2-way ANOVA compared to control. (D) Average difference compared to control condition for all coronal brain slices analyzed. One-way ANOVA compared to control. (E) Timeline diagram of experiment. Arrows on the bottom indicate alcohol (EtOH) or saline injections. This experiment was performed 24 hours after the last of 30 daily tracer injections. (F) Tracer influx in the cortex 30 minutes after cisterna magna injections of Alexa-647-conjugated bovine serum albumin (BSA-647) in awake mice with 24 hours withdrawal from chronic alcohol treatment. Scale bars: 100 μm. (G) Quantifications of tracer influx in mice treated with alcohol or saline for 30 days, measured 24 hours after the last treatment. 2-way ANOVA compared to control. (H) Average difference compared to control for all coronal brain slices analyzed. One-way ANOVA compared to control. CTR, saline; low, 0.5 g/kg ethanol; medium, 1.5 g/kg ethanol. *p < 0.05, **p < 0.01, ***p < 0.001. Bar graphs represent mean and standard error of the mean (SEM) of 7–11 mice per group.
