Figure 3
Tracer clearance is improved in alcohol-treated mice. (A) CSF tracer in coronal slices of left brain hemisphere at 180 min after injection in mice chronically treated with saline (CTR), low (0.5 g/kg) and medium (1.5 g/kg) doses of alcohol with the last dose of alcohol given immediately before tracer injection (EtOH), (B) quantification of tracer in slices at the indicated distances to bregma (2-way ANOVA) and (C) average difference compared to control condition for all coronal brain slices analyzed (one-way ANOVA). (D) CSF tracer in coronal slices of left brain hemisphere at 180 min after injection in mice chronically treated with saline (CTR), low and medium dose of alcohol with the last dose of alcohol given 24 hours before tracer injection (withdrawal), (E) quantification of tracer in slices at the indicated distances to bregma (2-way ANOVA) and (F) average difference compared to control condition for all coronal brain slices analyzed (one-way ANOVA). *p < 0.05, **p < 0.01, ***p < 0.001. Bar graphs represent mean and standard error of the mean (SEM) of 5 saline mice, 10 low and 10 medium alcohol dose mice per group for both EtOH and withdrawal experiments.
