Figure 5
Chronic administration of 0.5 g/kg alcohol decreases GFAP, while 1.5 g/kg alcohol increases astrogliosis. Immunostaining for GFAP (astrocyte marker, magenta) and DAPI (white) and quantifications thereof in control mice and in mice chronically treated with alcohol in cortex (A,B), corpus callosum (C,D) and hippocampus (E,F). CTR, saline; low, 0.5 g/kg ethanol; medium, 1.5 g/kg ethanol. *p < 0.05, **p < 0.01, one-way ANOVA with Tukey test. Scale bars, 100 µm. Bar graphs represent mean and standard error of the mean (SEM) of 3–4 mice per group.
