Figure 2 | Scientific Reports

Figure 2

From: Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2

Figure 2

rMtb9.8 induces the secretion of TNF-α by THP-1 cells. THP-1 cells were cultured in the presence of rMtb9.8 (5 µg/ml) for 4 h at 37 °C. The cell culture supernatant was collected, and the level of TNF-α in the supernatant was measured using ELISA. Pam3CSK4 (10 µg/ml), a TLR1/2 agonist, was used as a positive control. The pET30 vector tag protein without the Mtb9.8 gene (PET) was used as a negative control. Proteinase K abrogated rMtb9.8-induced expression of TNF-α (p < 0.001), as described above. rMtb9.8 contaminated by LPS may contribute to the expression of TNF-α; therefore, polymyxin B (PB), an inhibitor of LPS, was used.

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