Figure 3 | Scientific Reports

Figure 3

From: Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2

Figure 3

TLR2 is required for the secretion of TNF-α and IL-1β by rMtb9.8-induced mouse macrophages. RAW264.7 cells were pre-treated for 30 min at 37 °C with anti-TLR2 (30 µg/ml), anti-TLR4 (30 µg/ml) or isotype Ab (30 µg/ml) before stimulation with rMtb9.8 (5 μg/ml) for 30 min. LPS and Pam3CSK4 were used as positive controls to demonstrate the blocking effects of TLR4 and TLR2 antibodies. The levels of TNF-α (A) and IL-1β (B) in the supernatant were measured by ELISA. The data are expressed as the mean ± SD of the results of three separate experiments. **P < 0.01, ***P < 0.001, stimulated cells versus cells cultured in medium alone.

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