Figure 7 | Scientific Reports

Figure 7

From: Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2

Figure 7

rTB9.8 induces the nuclear translocation of IRF-1. RAW264.7 cells were cultured in the presence of rTB9.8 (5 µg/ml) for the indicated times, and cell lysates of total and nuclear proteins were prepared. Western blot analysis was used to examine the nuclear translocation of IRF-1. The cells were also stimulated with IFN-γ (10 U/ml), a known potent inducer of IRF-1 expression, as a positive control. Bottom, reprobing with anti-TBP and anti-β-actin to ensure equal loading. No IRF-1 protein was detectable in unstimulated cells at any of the time points examined. The data shown represent one of three independent experiments. The full-length blots are presented in Supplementary Figure 1.

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