Figure 4 | Scientific Reports

Figure 4

From: Electrophilic fatty acid nitroalkenes regulate Nrf2 and NF-κB signaling:A medicinal chemistry investigation of structure-function relationships

Figure 4

Dose-dependent inhibition of TNFα-induced NF-kB luciferase activity. Stable HEK293cells were co-treated for 2 hours with 100 pg/mL TNFα (to induced NF-κB activity) and with increasing concentrations at 2.5, 5 and 10 μM for all electrophilic compounds with the exception of CDDO-Im (10, 50 and 100 nM). Within an individual experiment, there was a minimum of an n = 4/treatment which was normalized to the 100 pg/mL TNFα treatment in media. The values in the bar graph represent mean ± SEM for at least three independent experiments (n = 3–6). Compared to the vehicle (DMSO) treatment group, statistical significance is indicated by: (a) p < 0.05 and (c) p < 0.001.

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