Table 2 Biophysicals properties of WT, R1451C and R1451L channels at 10 °C.

From: Substitutions of the S4DIV R2 residue (R1451) in NaV1.4 lead to complex forms of paramyotonia congenita and periodic paralyses

 

WT

R1451C

R1451L

Mean ± sem

n

Mean ± sem

n

Mean ± sem

n

Steady-state activation

 

11

 

8

 

9

  V 1/2

−22.5 ± 1.6

 

−16.5 ± 2.7*

 

−14.7 ± 1.3**

 

  k v

−8.4 ± 0.2

 

−10.6 ± 0.3***

 

−12.4 ± 0.7***

 

Steady-state inactivation

 

10

 

8

 

7

  V 1/2

−79.7 ± 1.4

 

−86.4 ± 2.8*

 

−97 ± 1.2***

 

  k v

5.7 ± 0.3

 

10 ± 0.9***

 

10.2 ± 0.2***

 

Recovery from fast inactivation

 

5

 

8

 

6

τslow

4.4 ± 0.2

 

4.3 ± 0.3

 

3.3 ± 0.3*

 
  1. V1/2, midpoint for activation or inactivation (mV); k, slope factor for activation or inactivation; τ, time constant (ms); A, fraction of the τ components (%); n, number of cells. Values are means ± sem, *P < 0.05, **P < 0.001, ***P < 0.001, data were significantly different for mutant channels when compared to WT at 22 °C.
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