Figure 5
Functional expression of WT or Q913X mutants in Xenopus oocytes. (A) HCO3−-dependent Gm defined as Gm in ‘Na, HCO3’ solution less the Gm in ‘Na (0 HCO3)’ solution, pooled from Figs 2 and 4A. Groups that do not share the same annotated letter are deemed significantly different by ANOVA with post hoc Tukey analysis, 95% confident limit. (B) A representative anti-EGFP western blot of isolated membrane (biotinylated) fractions from Xenopus oocytes injected with H2O, or cRNA encoding WT or a Q913X mutant. (C) Bar chart of average EGFP intensity signal from western blot of biotinylated fractions of Q913X-expressing oocytes (black bars), such as that in Fig. 5B, normalized to the signal from WT-expressing oocytes from each blot. Data are plotted alongside a similarly normalized version of the data presented in Fig. 5A (gray bars). ‘ns’ denotes no significance between bars (P > 0.007, accounting for Bonferroni correction for seven comparisons).
