Figure 9

Distribution of WT or Q913X mutant NBCe1-A-EGFP in polarised MDCK−II Cells. (A) Representative WT transfected cells showing the distribution of NBCe1-EGFP and Na⁺-K⁺ ATPase in the XY, XZ, and YZ plane as disclosed by an anti-EGFP primary antibody following by an Alexa-488 conjugated secondary antibody (green) and anti-Na⁺-K⁺ ATPase followed by an Alexa-594 conjugated secondary antibody (red). (B–G) Equivalent representative from cells transfected with Q913R, Q913C, Q913E, Q913K, Q913L, and Q913S. (H) Bar chart showing the average Pearson’s coefficient values for co-incidence of EGFP immunoreactivity with Na⁺-K⁺ ATPase immunoreactivity. Groups that do not share the same annotated letter are deemed significantly different by ANOVA with post hoc Tukey analysis, 95% confident limit.