Table 2 Primers used for the Quantitative Polymerase Chain Reaction (qPCR).

From: Effect of tetramethylpyrazine on tibial dyschondroplasia incidence, tibial angiogenesis, performance and characteristics via HIF-1α/VEGF signaling pathway in chickens

Genes

Accession number

Primer sequence (5′-3′)

Product size (bp)

Tm (°C)

VEGF

XM_019612783

F: AAAGCGAGGAAAGGGGAAGG

94

55

R: TCTCCTCTCTGAGCAAGGCT

HIF-1a

Q16665

F: TGAGAGAAATGCTTACACACAG

263

56

R: TGATGGGTGAGGAATTGGTTCAC

GAPDH

XM_019960295

F: CCTTCATTGACCTTCACTACATGGTCTA

127

58

R: TGGAAGATGGTGATGGCCTTTCCATTG

Back to article page