Figure 1
From: TDP-43 self-interaction is modulated by redox-active compounds Auranofin, Chelerythrine and Riluzole
TDP-43 self-interaction. NanoBit® luciferase complementation assay for protein interactions has been used to measure interaction of different N-terminal large (LgBit, pFN33), small (SmBit, pFN35), C-terminal large (LgBit, pFC34) and small (SmBit, pFC36) fusion proteins of TDP-43, positive control vectors SmBiT-PRKACA and LgBiT-PRKAR2A (coding the catalytic and regulatory subunits of PKA) and vector SmBiT-Halotag, which contains haloalkane dehalogenase – smBit fusion protein (Halitag) as a negative control, were obtained from Promega (Madison, WI, USA) and have been used in parallel 24 h after transfection of N2a cells and seeding cells into 384-well plates, Mean ± SD, Mann-Whitney-U-Test **p < 0.01.
