Figure 2
From: TDP-43 self-interaction is modulated by redox-active compounds Auranofin, Chelerythrine and Riluzole
Flowchart of NanoBit luciferase measurement with LOPAC®1280-screen. Description of measurement procedure with compounds from LOPAC®1280 library. For reduction of variability, luciferase reading from each well after treatment has been normalized to the same well before treatment. To detect off-target effects of compounds such as interference with luciferase reaction, protein homeostasis and TK promoter activity, we applied the same compounds onto N2a cells transfected with a TK-promoter construct expressing full length NanoLuc luciferase. Therefore, results of TDP-43 self-interaction were normalized with respect to NanoLuc activity after library treatment.
